Beacon Designer 5.10
Beacon Designer helps design successful SYBR Green, TaqMan, LNA spiked TaqMan, molecular beacon for both standard and NASBA assays and FRET assays by interpreting BLAST results for specificity and locating oligos avoiding template structures.
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Beacon Designer helps design successful SYBR Green, TaqMan, LNA spiked TaqMan, molecular beacon for both standard and NASBA assays and FRET assays by interpreting BLAST results for specificity and locating oligos avoiding template structures.Beacon Designer is a tool that allows you calculate and edit primers.
Beacon Designer features
SYBR Green Primer DesignTaqMan Design
Beacon Designer designs LNA or Locked Nucleic Acid substituted TaqMan probes to improve real-time PCR assay success.
Tm adjustment- Automatically selects a stem of appropriate length for optimal beacon Tm.
NASBA assays- short for Nucleic Acid Sequence Based Amplification, is gaining acceptance as an isothermal amplification process. It amplifies mRNA in double-stranded DNA without temperature cycling. Beacon Designer can design molecular beacon probes for NASBA assays for single template, multiplex or alleleic discrimination.
Optimal FRET probes- Designs optimal FRET probes free of dimers, repeats and runs to ensure signal fidelity.
BLAST search- Performs BLAST search on a selected sequence, primers and amplicon.
Database and Data Management
Projects- Allows creation of multiple projects. A large amount of data can be easily organized and managed by creating a separate project for each experiment.
Web Integration
Web Entrez- Quickly retrieves batches of sequences from Entrez using accession numbers.
Input/Output
Generate Report- You will now be able to create an attractively formatted report for the assays you designed. It should be helpful in record keeping and for sharing information with colleagues. The report helps visualize the positions of the primers and probes on the sequence, includes a list of the alternate primers and probes, displays primers, probe, amplicon and sequence properties and the design parameters used.
tags pre designed beacon designer primers and and probes probe can taqman probes nasba assays blast search designed primer primers for secondary structures primer design molecular beacon
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Authors software
Beacon Designer 5.10
Premier Biosoft International
Beacon Designer helps design successful SYBR Green, TaqMan, LNA spiked TaqMan, molecular beacon for both standard and NASBA assays and FRET assays by interpreting BLAST results for specificity and locating oligos avoiding template structures.
Primer Premier 5.00
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Primer Premier is a comprehensive primer design application.
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